Contact:

Dirk Dormann

Head of Microscopy

t:02083838528

e:dirk.dormann@lms.mrc.ac.uk

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

WIDEFIELD MICROSCOPES

 

ZEISS Upright

ZEISS UPRIGHT

Conventional upright microscope used for high resolution colour imaging. Optional circular polarization optics.

Microscope

Manual Zeiss Axiophot with phase contrast and DIC for transmitted light illumination.

Objectives

Detectors

Software

Accessories

Location

room 5030b/CRB

 

LEICA FISH

LEICA FISH

Conventional upright microscope used for high resolution fluorescence and brightfield colour imaging.

Microscope

Manual Leica DMRB with phase contrast and DIC for transmitted light illumination. Individual DAPI, FITC and TRITC filter sets for conventional epifluorescence illumination with 100W HBO lamp. Also with Penta dichroic for DAPI/FITC/TRITC/Cy5/Cy-7 and Ludl filterwheel for automated excitation filter selection.

Objectives

Detectors

Software

Location

room 5027/CRB

 

OLYMPUS Stereo microscope

OLYMPUS STEREO

Stereo microscope for colour and fluorescence imaging of large samples.

Microscope

Manual Olympus SMZ-16X microscope with white LED transmitted light source, optional dark field and oblique illumination. Schott cold light source and optional Tokai Thermoplate heated stage. DAPI, GFP, TRITC and TexasRed filter sets for conventional epifluorescence illumination.

Objectives

Detectors

Software

Accessories

Location

room 2020/CRB

 

LEICA Microinjection

MICROINJECTION

Inverted microscope often used for examining GFP/RFP transfected cells in multiwell plates or Petri dishes, with sensitive fluorescence camera and separate colour camera. Equipped with Eppendorf Microinjector.

Microscope

Motorised Leica DMIRE2 stand with phase contrast for transmitted light illumination. Individual DAPI, FITC and TRITC filter sets for conventional epifluorescence illumination with 100W HBO lamp. Eppendorf Microinjector/-manipulator.

Objectives

Detectors

Software

Accessories

Location

room 5032b/CRB

 

DELTAVISION Elite

DELTAVISION

Widefield fluorescence live imaging system with high precision motorized XYZ stage for automated multi-position experiments and 488nm laser for FRAP studies. Lumencor Spectra LED light source. Dual camera setup with high resolution camera for fixed samples and high sensitivity EMCCD camera for live imaging. Following acquisition image stacks can be deconvolved to remove out-of-focus light and enhance contrast.

Microscope

Manual inverted Olympus IX71 with DIC for transmitted light illumination.

Filtersets

Laser lines

Objectives

Detectors

Software

Location

room 5027/CRB

 

OLYMPUS IX70

OLYMPUS IX70

Widefield fluorescence live imaging system with high precision motorized ASI XYZ stage for automated multi-position experiments. Lumencor Spectra LED light source and Sutter emission filter wheel. Colour DSLR camera for slide scanning./p>

Microscope

Manual inverted Olympus IX70 with phase contrast and optional circular polarisation optics for transmitted light illumination.

Filtersets

Objectives

Detectors

Software

Location

room 5027/CRB

 

Ultraview Spinning Disk confocal and widefield imaging system

Ultraview

Spinning disk confocal (488nm laser only !) and widefield system for live cell imaging.

Microscope

Manual Zeiss Axiovert 200 inverted microscope with phase contrast for transmitted light illumination. DAPI, GFP and DsRed filter sets for conventional epifluorescence illumination with Coolled LED. Fast 100┬Ám Piezo Z focus drive and ASI motorized stage. Yokogawa CSU-10 scan head.

Objectives

Laser lines

Detectors

Software

Accessories

Location

room 3031b/CRB

 

ZEISS Deconvolution

DECONVOLUTION

Widefield fluorescence imaging system with motorized Z focus control and automated shutter and filterwheels.

Available also to Imperial College users !

Microscope

Automated inverted Zeiss Axiovert 200M with Phase contrast. Quadruple dichroic with DAPI/FITC/TRITC/Cy5 filters for epifluorescence illumination with 100W HBO lamp. Sutter filterwheel and shutters.

Filtersets

Objectives

Detectors

Software

Location

room BS17/CWB